Activities – Ishima Lab

Zhaoyong Xi and Tatiana Ilina published a paper, The reduced form of the antibody CH2 domain., on Protein Science
Zhaoyong Xi and Tatiana Ilina published their paper, Relative domain orientation of the L289K HIV-1 reverse transcriptase monomer“, on Protein Science
We published a review article, Retroviral RNase H: Structure, mechanism, and inhibition., Enzymes. 2021;50:227-247. PMID: 34861939

Shahid Khan and John Persons published their paper, “A synergy of activity, stability, and inhibitor-interaction of HIV-1 protease mutants evolved under drug-pressure.” ,on Protein Science
Tatiana Ilina published her paper, “Effect of Lysyl-tRNA Synthetase on the Maturation of HIV-1 Reverse Transcriptase“, on ACS Omega
Zhaoyong Xi published her paper, “Determinants of Active-Site Inhibitor Interaction with HIV1 RNase H“, on ACS Infectious Diseases.
We also wrote an interesting review article. Ilina TV, Xi Z, Brosenitsch TA, Sluis-Cremer, N, Ishima R. Large multidomain protein NMR: HIV-1 reverse transcriptase precursor in solution, Int J Mol Sci, 2020Dec, in press, PMID: 33333923.
As you can imagine, our group did not have much outside activity in 2020…

Congratulations to Ryan Slack on his successful PhD defense on December 14.
Tatiana Ilina attended and presented her poster at the Frontiers in Retrovirology Conference (Sep 11-13) in Leuven, Flanders, Belgium
Rieko Ishima presented in Dr. Mike Parniak Memorial Symposium on September 7, at University of Pittsburgh School of Medicine, Pittsburgh, PA
Shahid Khan and John Persons published their paper, “Probing Structural Changes among Analogous Inhibitor-Bound Forms of HIV-1 Protease and a Drug-Resistant Mutant in Solution by Nuclear Magnetic Resonance.” ,on Biochemistry.
John Persons and Shahid Khan published their paper, “An NMR strategy to detect conformational differences in a protein complexed with highly analogous inhibitors in solution” , on Methods.
Tatiana Ilina published her paper, “Effect of tRNA on the Maturation of HIV-1 Reverse Transcriptase” , on J Mol Biol.
Chunyan Liu, Associate Chief Physician from Tianjin Medical University General Hospital in China, worked for one year as her sabbatical in our group.

Ryan Slack presented his poster, “Structural characteristics of the RNase H domain in HIV-1 Reverse Transcriptase”, in the 61th Annual meeting of Biophysics Society (Feb 11-15) in New Orleans, LA.
Tatiana Ilina presented her poster, “Effect of tRNA on the HIV-1 reverse transcriptase maturation”, in the 41st annual meeting on Retroviruses at Cold Spring Harbor (May 22-27), Cold Spring Harbor, NY.
Kylee Karczewski in Allegheny College joined our group as a summer student. She presented her reserach in the SURP closing day presentation. The title of her talk was “Biophysical Characterization of Mutants of HIV-1 RT Precursor” on July 28
Rieko ishima served as a co-session chair for Reverse transcription in the 41st annual meeting on Retroviruses at Cold Spring Harbor (May 22-27), Cold Spring Harbor, NY.

Ichhuk Karki published his paper, “Entire-Dataset Analysis of NMR Fast-Exchange Titration Spectra: A Mg2+ Titration Analysis for HIV-1 Ribonuclease H Domain” ,on J Phys Chem B.
Ichhuk Karki and John Persons attended and presented their posters in the 57th Experimenetal NMR Conference in Pittsburgh, PA (Apr 9 – 14).
Tatiana Ilina presented her talk, “tRNA-mediated maturation of HIV-1 reverse transcriptase”, in the 40th annual meeting on Retroviruses at Cold Spring Harbor (May 23-28), Cold Spring Harbor, NY.
Zhaoyong Xi and Shahid Khan attended the XXVII International Conference on Magnetic Resonance in Biological Systems (Aug 21-26) in Kyoto, Japan, and presented their posters.
Masayuki Oda, Professor from Kyoto Prefectural University, worked during the summer as his sabbatical in our group.

Ryan Slack published his paper, “Structural Integrity of the Ribonuclease H domain in HIV-1 Reverse Transcriptase” , on Proteins.
Ryan Slack gave a talk in the Gordon Research Symposium (June 6-7) and presented his poster in the Gordon Research Conference (June 7-12) in Italy, Lucca.
Ichhuk Karki, Shahid Khan, John Persons, Kenji Kanaori attended NIGMS Structural Biology related HIV/AIDS meeting in Bethesda, MD, June 18-19.
Kanaori Kenji, sabbatical Professor from Kyoto Institute of Technology, Japan, in my group gave a talk in the Center for Nucleic Acids Science and Technology (CNAST) in Carnegie Mellon University on September 15.
John Persons visited Institute of Protein Research in Osaka Universiy , Japan, for his international collaboration work from November 6 to 17.